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wheat germ agglutinin wga  (Vector Laboratories)


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    Structured Review

    Vector Laboratories wheat germ agglutinin wga
    Wheat Germ Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wga+rhodamine/Rhodamine+labeled+Wheat+Germ+Agglutinin+(WGA)/pm42037405-280-9-17
    Average 96 stars, based on 268 article reviews
    wheat germ agglutinin wga - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Binding Assay:

    Article Title: The microenvironment dictates glycocalyx construction and immune surveillance
    Article Snippet: .. Lectins/carbohydrate binding reagents: SNA-biotin (Vector, B-1305–2), GNL-biotin (Vector, B-1245-2), and WGA-rhodamine (Vector, RL-1022). .. SLBR-N-biotin was generated by expressing the glutathione S-transferase (GST)-NCTC10712BR fusion protein in Escherichia coli (strain BL21(DE3)) and purified using glutathione Sepharose Concentrated stock solutions [2 mg/mL] of SLBR-N were modified with Biotin via, EZ-Link NHS-Biotin (ThermoFisher, 20217), followed by thorough PBS dialysis at 4 °C, and then used as a staining reagent.

    Article Title: The microenvironment dictates glycocalyx construction and immune surveillance
    Article Snippet: APTES activated coverslips were coated with PBS buffered acrylamide / bis-acrylamide (Bio-Rad, 1610140 and 1610142) solutions (3% / 0.05% for 400 Pa, 7.5% / 0.07% for 6k Pa, and 10% / 0.5% for 60k Pa) polymerized with TEMED (0.1% v/v) (Bio-Rad, 1610801) and Potassium Persulfate (0.1% w/v) (Fisher, BP180) to yield a final thickness of ~ 85 μm. .. Lectins/carbohydrate binding reagents: SNA-biotin (Vector, B-1305–2), GNLbiotin (Vector, B-1245-2), and WGA-rhodamine (Vector, RL-1022). .. DOPA coated PA-gels were washed 2x with sterile PBS and ECM functionalized with 5 μg/mL human fibronectin (Millipore, FC010) in sterile PBS 1 h at 37 oC to generate an expected fibronectin coating density of 6 μM/cm2.

    Whole Genome Amplification:

    Article Title: The microenvironment dictates glycocalyx construction and immune surveillance
    Article Snippet: .. Lectins/carbohydrate binding reagents: SNA-biotin (Vector, B-1305–2), GNL-biotin (Vector, B-1245-2), and WGA-rhodamine (Vector, RL-1022). .. SLBR-N-biotin was generated by expressing the glutathione S-transferase (GST)-NCTC10712BR fusion protein in Escherichia coli (strain BL21(DE3)) and purified using glutathione Sepharose Concentrated stock solutions [2 mg/mL] of SLBR-N were modified with Biotin via, EZ-Link NHS-Biotin (ThermoFisher, 20217), followed by thorough PBS dialysis at 4 °C, and then used as a staining reagent.

    Article Title: Divergence in skeletal muscle growth by differential spatial hyperplastic patterning in teleost fishes
    Article Snippet: Next, sections were washed into PBST, then incubated in secondary antibodies and dyes diluted in corresponding blocking solutions for 4h (cryosections) or overnight at 4°C (for vibratome sections) respectively, then washed into PBST. .. The following dyes and secondary antibodies were used: DAPI (1:300), Phalloidin 555 (1:400, Invitrogen, A30106), Phalloidin 647 (1:400, Invitrogen, A22287), WGA-fluorescein (1:300, VectorLabs, FL-1021-5), WGA-rhodamine (1:300, VectorLabs, RL-1022-5) and various Alexa Fluor antibodies (Invitrogen) targeting corresponding primary antibodies. .. For imaging, all vibratome sections were mounted in 1% low melt agarose plates and imaged via Leica SP8 multiphoton (confocal) microscope controlled by Las X software.

    Article Title: The AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells.
    Article Snippet: .. Then, sections were incubated with YAP (Cell Signaling Technology, #14074, 1:200) antibody overnight at 4 °C, followed with the appropriate secondary antibody and WGA-Rhodamine (Vector Laboratories, RL-1022, 1:200). ..

    Article Title: fishROI: A specialized workflow for semi-automated muscle morphometry analysis in teleosts
    Article Snippet: .. The tissue was then incubated in 2% goat serum block for 30 min, followed by incubation with WGA-Rhodamine (VectorLabs, RL1022, 1:300 dilution) for 2 hours, followed by 6 PBST washes. .. Sample was subsequently mounted in 80% glycerol in PBST and imaged on the Leica thunder deconvolution microscope.

    Article Title: Mef2d Acts Upstream of Muscle Identity Genes and Couples Lateral Myogenesis to Dermomyotome Formation in Xenopus laevis
    Article Snippet: .. Stage 13 embryos were injected using pulled-glass capillaries either with WGA-rhodamine (dissolved at 200 μg/ml, Vector laboratories) to be analysed at stage 18 or with both WGA-rhodamine and -fluorescein (Vector laboratories) to be analysed at stage 23. ..

    Article Title: The microenvironment dictates glycocalyx construction and immune surveillance
    Article Snippet: APTES activated coverslips were coated with PBS buffered acrylamide / bis-acrylamide (Bio-Rad, 1610140 and 1610142) solutions (3% / 0.05% for 400 Pa, 7.5% / 0.07% for 6k Pa, and 10% / 0.5% for 60k Pa) polymerized with TEMED (0.1% v/v) (Bio-Rad, 1610801) and Potassium Persulfate (0.1% w/v) (Fisher, BP180) to yield a final thickness of ~ 85 μm. .. Lectins/carbohydrate binding reagents: SNA-biotin (Vector, B-1305–2), GNLbiotin (Vector, B-1245-2), and WGA-rhodamine (Vector, RL-1022). .. DOPA coated PA-gels were washed 2x with sterile PBS and ECM functionalized with 5 μg/mL human fibronectin (Millipore, FC010) in sterile PBS 1 h at 37 oC to generate an expected fibronectin coating density of 6 μM/cm2.

    Article Title: YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells
    Article Snippet: .. Then, sections were incubated with YAP (Cell Signaling Technology, #14074, 1:200) antibody overnight at 4°C, followed with the appropriate secondary antibody and WGA-Rhodamine (Vector Laboratories, RL-1022, 1:200). ..

    Article Title: The AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells
    Article Snippet: .. Then, sections were incubated with YAP (Cell Signaling Technology, #14074, 1:200) antibody overnight at 4 °C, followed with the appropriate secondary antibody and WGA-Rhodamine (Vector Laboratories, RL-1022, 1:200). ..

    Incubation:

    Article Title: The AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells.
    Article Snippet: .. Then, sections were incubated with YAP (Cell Signaling Technology, #14074, 1:200) antibody overnight at 4 °C, followed with the appropriate secondary antibody and WGA-Rhodamine (Vector Laboratories, RL-1022, 1:200). ..

    Article Title: fishROI: A specialized workflow for semi-automated muscle morphometry analysis in teleosts
    Article Snippet: .. The tissue was then incubated in 2% goat serum block for 30 min, followed by incubation with WGA-Rhodamine (VectorLabs, RL1022, 1:300 dilution) for 2 hours, followed by 6 PBST washes. .. Sample was subsequently mounted in 80% glycerol in PBST and imaged on the Leica thunder deconvolution microscope.

    Article Title: YAP-dependent autophagy is controlled by AMPK, SIRT1 and flow intensity in kidney epithelial cells
    Article Snippet: .. Then, sections were incubated with YAP (Cell Signaling Technology, #14074, 1:200) antibody overnight at 4°C, followed with the appropriate secondary antibody and WGA-Rhodamine (Vector Laboratories, RL-1022, 1:200). ..

    Article Title: The AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells
    Article Snippet: .. Then, sections were incubated with YAP (Cell Signaling Technology, #14074, 1:200) antibody overnight at 4 °C, followed with the appropriate secondary antibody and WGA-Rhodamine (Vector Laboratories, RL-1022, 1:200). ..

    Blocking Assay:

    Article Title: fishROI: A specialized workflow for semi-automated muscle morphometry analysis in teleosts
    Article Snippet: .. The tissue was then incubated in 2% goat serum block for 30 min, followed by incubation with WGA-Rhodamine (VectorLabs, RL1022, 1:300 dilution) for 2 hours, followed by 6 PBST washes. .. Sample was subsequently mounted in 80% glycerol in PBST and imaged on the Leica thunder deconvolution microscope.

    Injection:

    Article Title: Mef2d Acts Upstream of Muscle Identity Genes and Couples Lateral Myogenesis to Dermomyotome Formation in Xenopus laevis
    Article Snippet: .. Stage 13 embryos were injected using pulled-glass capillaries either with WGA-rhodamine (dissolved at 200 μg/ml, Vector laboratories) to be analysed at stage 18 or with both WGA-rhodamine and -fluorescein (Vector laboratories) to be analysed at stage 23. ..



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